Unit 6: Gene Expression and Regulation

Based on the mRNA sequence, what is the amino acid sequence of the resulting polypeptide?

During translation, the mRNA codon is read by a complementary

If a point mutation changes the third codon from UAC to UAG, what is the effect on the polypeptide?

If a single nucleotide insertion occurs after the AUG start codon, the most dramatic effect would be

During transcription, which enzyme synthesizes mRNA from a DNA template?

In eukaryotes, the removal of introns from pre-mRNA is carried out by

Alternative splicing allows a single gene to

The lac operon in E. coli is an example of

Which of the following correctly pairs a type of mutation with its description?

Epigenetic modifications such as DNA methylation can affect gene expression by

A researcher uses gel electrophoresis to separate DNA fragments produced by restriction enzyme digestion. The DNA fragments migrate through the gel based on

Which of the following technologies allows scientists to edit specific genes in living organisms?

The redundancy (degeneracy) of the genetic code means that

In the PCR process described, what is the purpose of the 95°C denaturation step?

After 30 cycles of PCR, approximately how many copies of the target DNA would be produced?

Taq polymerase is used in PCR instead of human DNA polymerase because

A DNA strand reads 3'-TACGAATCG-5'. What is the corresponding mRNA sequence?

If a gene contains 900 nucleotides in the coding region (excluding the stop codon), how many amino acids will the resulting polypeptide contain?

RNA interference (RNAi) regulates gene expression by

Which of the following best explains why β-galactosidase is produced at high levels only in condition 2 (lactose-only medium)?